While Illumina ultimately opted for a method that simultaneously detected massive numbers of DNA strands, others still believed that single-molecule sequencing methods offered a better path forward. Sequencing by synthesis requires repeated amplification, which introduces the possibility of error at each step and biases outputs towards sequences readily amplified by DNA polymerase. Single-molecule techniques were billed as a way to sequence DNA with minimal bias while simultaneously reducing cost.
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evaluation-order dependence, though.
I hate DRM, I love FOSS, I love the very idea that the internet should be open and accessible.，这一点在体育直播中也有详细论述